The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 11, 2013

Filed:

Feb. 28, 2008
Applicants:

Tadashi Fujii, Tokyo, JP;

Yoshikazu Fujii, Tokyo, JP;

Atsushi Ochiai, Tokyo, JP;

Masashi Ito, Tokyo, JP;

Kazuhiro Machida, Tokyo, JP;

Inventors:

Tadashi Fujii, Tokyo, JP;

Yoshikazu Fujii, Tokyo, JP;

Atsushi Ochiai, Tokyo, JP;

Masashi Ito, Tokyo, JP;

Kazuhiro Machida, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

Disclosed is a means for improving the poor conversion efficiency in a bioconversion system using ancell having a bacterium-originated cytochrome P-450 gene integrated therein. A recombinantcell is produced by introducing aciB and aciC which encode a gene for the electron transport system originated from thesp. OC4 strain into ancell, and adding a polynucleotide encoding an N-terminal sequence composed of 48 amino acid residues of AciA and the like to the 5'-terminus of a bacterium-originated cytochrome P-450 gene, wherein AciA is an alkane-oxidative cytochrome P-450 originated from thesp. OC4 strain. Use of the recombinantcell results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound.


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