The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 21, 2013
Filed:
May. 14, 2010
Paolo Vatta, San Mateo, CA (US);
Olga Petrauskene, San Carlos, CA (US);
Manohar Furtado, San Ramon, CA (US);
Pius Brzoska, Woodside, CA (US);
Lily Wong, Oakland, CA (US);
Melissa Barker, Gilroy, CA (US);
Craig A. Cummings, Pacifica, CA (US);
Paolo Vatta, San Mateo, CA (US);
Olga Petrauskene, San Carlos, CA (US);
Manohar Furtado, San Ramon, CA (US);
Pius Brzoska, Woodside, CA (US);
Lily Wong, Oakland, CA (US);
Melissa Barker, Gilroy, CA (US);
Craig A. Cummings, Pacifica, CA (US);
LIfe Technologies Corporation, Carlsbad, CA (US);
Abstract
Disclosed are primer and probe compositions, PCR assays, methods and kits for the specific detection ofO157:H7 and notO55:H7 from a variety of samples. In some embodiments, methods for specifically detectingO157:H7 comprise: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying said at least first and said at least second target polynucleotide sequences, and detecting said at least first and said at least second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence ofO157:H7 in a sample and notO55:H7.