The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 14, 2013
Filed:
Oct. 29, 2010
Thai V. Truong, Pasadena, CA (US);
John M. Choi, Tujunga, CA (US);
Scott E. Fraser, LaCanada, CA (US);
Willy Supatto, Paris, FR;
David S. Koos, Pasadena, CA (US);
Thai V. Truong, Pasadena, CA (US);
John M. Choi, Tujunga, CA (US);
Scott E. Fraser, LaCanada, CA (US);
Willy Supatto, Paris, FR;
David S. Koos, Pasadena, CA (US);
California Institute of Technology, Pasadena, CA (US);
Abstract
An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of wholeand zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.