The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 2013
Filed:
Jun. 27, 2008
Eric A. Johnson, Madison, WI (US);
Kristin M. Marshall, Plainfield, WI (US);
Sabine Pellett, Madison, WI (US);
Marite Bradshaw, Madison, WI (US);
Eric A. Johnson, Madison, WI (US);
Kristin M. Marshall, Plainfield, WI (US);
Sabine Pellett, Madison, WI (US);
Marite Bradshaw, Madison, WI (US);
Wisconsin Alumni Research Foundation, Madison, WI (US);
Abstract
The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in uniquetype A strains and encode either BoNT/A3 or BoNT/A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encodedneurotoxin andneurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from atype A strain. The inventors performed comparative analyzes of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/A3 in the A3 strain, and BoNT/A4 and BoNT/B in the A4 strain, are on plasmids.