The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 12, 2013
Filed:
Jul. 14, 2008
Zhenyu Zhu, Beverly, MA (US);
Aine Blanchard, Wakefield, MA (US);
Shuang-yong Xu, Lexington, MA (US);
Shengxi Guan, Ipswich, MA (US);
Hua Wei, Ipswich, MA (US);
Penghua Zhang, Lexington, MA (US);
Dapeng Sun, Arlington, MA (US);
Siu-hong Chan, Ipswich, MA (US);
Zhenyu Zhu, Beverly, MA (US);
Aine Blanchard, Wakefield, MA (US);
Shuang-Yong Xu, Lexington, MA (US);
Shengxi Guan, Ipswich, MA (US);
Hua Wei, Ipswich, MA (US);
Penghua Zhang, Lexington, MA (US);
Dapeng Sun, Arlington, MA (US);
Siu-hong Chan, Ipswich, MA (US);
New England Biolabs, Inc., Ipswich, MA (US);
Abstract
Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions include restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.