The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 29, 2013
Filed:
Mar. 10, 2009
Philippe Menasche, Paris, FR;
Michel Puceat, Champcueil, FR;
Jérôme Larghero, Boulogne-Billancourt, FR;
Guillaume Blin, Paris, FR;
David Nury, Corbeil, FR;
Sonia Stefanovic, Draveil, FR;
Philippe Menasche, Paris, FR;
Michel Puceat, Champcueil, FR;
Jérôme Larghero, Boulogne-Billancourt, FR;
Guillaume Blin, Paris, FR;
David Nury, Corbeil, FR;
Sonia Stefanovic, Draveil, FR;
Assistance Publique—Hopitaux de Paris, Paris, FR;
Institut National de la Sante et de la Recherche Medicale (Inserm), Paris, FR;
Universite Paris Descartes, Paris, FR;
Universite Paris Diderot Paris 7, Paris, FR;
Abstract
The present invention is directed to a method for the in vitro preparation of cardiovascular progenitors cells from mammalian embryonic stem cells (ES cells) or mammalian embryonic-like state cells, preferably from primate, wherein said method comprises the use of the CD15 (SSEAI) marker as a positive cardiovascular progenitors differentiation marker. The present invention also claimed the use of a receptor tyrosine kinase inhibitor, particularly the SU5402 or SU11248 in association with the BMP2 for improving the efficiency of the desired differentiation. The present invention is also directed to the use of platelet lysate as foetal animal serum substitute in a culture medium intended to the proliferation or propagation of primate ES cells maintaining their pluripotency feature. Derived compositions or kits in relation with the claimed methods or product obtainable by the claimed methods form also part of the present invention.