The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 30, 2012
Filed:
Dec. 24, 2004
Naoko Horikoshi, Tsuchiura, JP;
Susumu Kawasaki, Tsukuba, JP;
Yukio Okada, Tsuchiura, JP;
Kazuko Takeshita, Tsuchiura, JP;
Takashi Sameshima, Tsuchiura, JP;
Shinichi Kawamoto, Tsukuba, JP;
Kenji Isshiki, Tsukuba, JP;
Naoko Horikoshi, Tsuchiura, JP;
Susumu Kawasaki, Tsukuba, JP;
Yukio Okada, Tsuchiura, JP;
Kazuko Takeshita, Tsuchiura, JP;
Takashi Sameshima, Tsuchiura, JP;
Shinichi Kawamoto, Tsukuba, JP;
Kenji Isshiki, Tsukuba, JP;
Prima Meat Packers, Ltd., Tokyo, JP;
National Agriculture and Food Research Organization, Tsukuba-shi, JP;
Abstract
The present invention is to provide a multiple detection method that can detect contaminating microorganisms existing in foods, including pathogenicO157,andspp., with high sensitivity comparable or even superior to official methods, comprising the steps of amplifying a plural number of target genes with a single PCR reaction tube and analyzing the same. The following steps are performed consecutively: (A) a step of extracting DNA of the target microorganisms to be detected by treating with at least a lytic enzyme such as Achromopepidase and Lysozyme and/or bacteriocin having lytic activity such as Enterolysine, a surfactant and a protein denaturing agent; and (B) a step of mixing a specific primer to the target microorganisms to be detected to perform multiplex PCR. Further, it is preferable to add a step of culturing with a culture condition where 1 CFU/100 g microorganisms becomes 10.sup.3 CFU/ml or more after 18 to 48 h of culture, for example that the pH after culture becomes 5.1 or more, before the step of extracting DNA of the target microorganisms to be detected.