The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 24, 2012
Filed:
Jul. 24, 2007
Arnaud Gautier, Lausanne, CH;
Kai Johnsson, Lausanne, CH;
Maik Kindermann, Frankfurt, DE;
Alexandre Juillerat, Paris, FR;
Florent Beaufils, Rosenau, FR;
Arnaud Gautier, Lausanne, CH;
Kai Johnsson, Lausanne, CH;
Maik Kindermann, Frankfurt, DE;
Alexandre Juillerat, Paris, FR;
Florent Beaufils, Rosenau, FR;
Ecole Polytechnique Federale de Lausanne, Lausanne, CH;
Abstract
The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The substrates according of the invention are substituted cytosines of formula (I) wherein Ris an aromatic or a heteroaromatic group, or an optionally substituted unsaturated alkyl, cycloalkyl or heterocyclyl group with the double bond connected to OCH—; Ris a linker; and L is a label or a plurality of same or different labels. The invention further relates to methods of transferring label L from these substrates of formula (I) to ACTs and ACT fusion proteins. The system of ACT-compound of formula (I) is particularly suitable for double labelling studies together with the known system O-alkylguanine-DNA alkyltransferase (AGT)-benzylguanines.