The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 03, 2012
Filed:
Jan. 11, 2008
Anant Y. Patkar, San Diego, CA (US);
Subrata Sen, San Diego, CA (US);
Micheal L. Chappell, Lake Jackson, TX (US);
Anant Y. Patkar, San Diego, CA (US);
Subrata Sen, San Diego, CA (US);
Micheal L. Chappell, Lake Jackson, TX (US);
Dow AgroSciences, LLC., Indianapolis, IN (US);
Abstract
A method of preparing a recombinant polypeptide of interest includes fermenting a host cell being transformed with a recombinant expression system capable of bringing about secretion of a polypeptide of interest into the periplasm of said host cell. The polypeptide of interest is extracted from the periplasm by applying a continuous osmotic shock to the host cells contained in a fermentation medium. An apparatus for osmotically shocking cells includes a first reservoir containing cells in a first solution and a second reservoir containing a second solution, the first solution having a higher osmolarity than the second solution. A method for osmotically shocking cells using the first and second solutions is also disclosed. Also disclosed is a method of isolating a recombinant polypeptide of interest from a cell.