The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 03, 2012
Filed:
Dec. 13, 2010
Yanbin LI, Fayetteville, AR (US);
Yongcheng Liu, Blacksburg, VA (US);
Yanbin Li, Fayetteville, AR (US);
Yongcheng Liu, Blacksburg, VA (US);
The Board of Trustees of the University of Arkansas, Little Rock, AR (US);
Abstract
The present invention is directed to satisfying the need to detect microbial contamination of food products. The described bioseparator/bioreactor coupled with an optical/electrochemical biosensor was able to specifically detectO157:H7 from 8.8×10to 8.8×10CFU/ml in 2.5 hours without any enrichment. Using this invention, concentrations of S. Typhimurium ranging from 8.6×10to 8.6×10CFU/ml in pure culture were detected in 2 hours without any enrichment. The invention may also be used for the detection of S. Seftenberg, which has the same sensitivity as S. Typhimurium. Other pathogens such asand S. Heidleberg did not interfere with the detection. The optimum inner diameter of the 25 cm long column for the detection ofO157:H7 is 250 μm. The detection limit for other microbial pathogens may be controlled by changing the length of capillary columns, using higher concentration of the labeled antibodies, altering the flow rate and concentration of the substrate, and increasing the reaction temperature to 37° C.