The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 03, 2012
Filed:
Aug. 10, 2010
John G. K. Williams, Lincoln, NE (US);
Gregory R Bashford, Lincoln, NE (US);
Jiyan Chen, Lincoln, NE (US);
Dan Draney, Lincoln, NE (US);
Nara Narayanan, Greensboro, NC (US);
Bambi L. Reynolds, Lincoln, NE (US);
Pamela Sheaff, Omaha, NE (US);
John G. K. Williams, Lincoln, NE (US);
Gregory R Bashford, Lincoln, NE (US);
Jiyan Chen, Lincoln, NE (US);
Dan Draney, Lincoln, NE (US);
Nara Narayanan, Greensboro, NC (US);
Bambi L. Reynolds, Lincoln, NE (US);
Pamela Sheaff, Omaha, NE (US);
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Abstract
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.