The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 22, 2011
Filed:
Feb. 01, 2005
Minxue Zheng, Foster City, CA (US);
David Ahle, Oakland, CA (US);
Brian Warner, Martinez, CA (US);
Ming Wu, Redwood City, CA (US);
Chu-an Chang, Piedmont, CA (US);
Minxue Zheng, Foster City, CA (US);
David Ahle, Oakland, CA (US);
Brian Warner, Martinez, CA (US);
Ming Wu, Redwood City, CA (US);
Chu-an Chang, Piedmont, CA (US);
Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US);
Abstract
The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal sequences may be used in singleplex or multiplex bDNA assays quantitatively and qualitatively to determine mRNA levels in a sample; to screen for and genotype targets, such as viruses, that are present in low volumes in a sample; to screen for and genotype SNPs; and to measure changes in the amount of a gene in a sample such as when gene amplifications or deletions occur. The highly orthogonal universal sequences may also be used as universal capture probes to selectively bind assay components in a way that facilitates their further analysis.