The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 22, 2011

Filed:

Dec. 21, 2006
Applicants:

Robert W. Mallett, Ravensdale, WA (US);

Christopher J. Stenland, Stanwood, WA (US);

Jonathon C. Boone, Kenmore, WA (US);

John W. Forstrom, Seattle, WA (US);

Karen S. DE Jongh, Seattle, WA (US);

Inventors:

Robert W. Mallett, Ravensdale, WA (US);

Christopher J. Stenland, Stanwood, WA (US);

Jonathon C. Boone, Kenmore, WA (US);

John W. Forstrom, Seattle, WA (US);

Karen S. De Jongh, Seattle, WA (US);

Assignee:

Zymogenetics, Inc., Princeton, NJ (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/74 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.


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