The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 2011

Filed:

Feb. 28, 2008
Applicants:

Yuji Ishida, Iwata, JP;

Yukoh Hiei, Iwata, JP;

Inventors:

Yuji Ishida, Iwata, JP;

Yukoh Hiei, Iwata, JP;

Assignee:

Japan Tobacco Inc., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01H 1/00 (2006.01); C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
Abstract

A novel-mediated method for producing a transformed monocotyledonous plant is provided. The transformation method involves (i) a coculture step for culturing an-inoculated monocotyledonous plant tissue with a coculture medium containing 3,6-dichloro-o-anisic acid, 4-amino-3,5,6-trichloropicolinic acid and/or 2,4,5-trichlorophenoxyacetic acid, and (ii) a regeneration step for culturing the tissue obtained in (i) with a regeneration medium containing a selective drug to thereby induce regeneration and to produce a transformed plant. The method does not include, between the coculture step and the regeneration step, any selection step for culturing the cocultured tissue with a medium containing an auxin and a selective drug to select a transformant.


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