The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 05, 2011
Filed:
Aug. 12, 2004
Thomas Meier, Munich, DE;
Waltraud Ankenbauer, Penzberg, DE;
Annette Deufel, Munich, DE;
Dieter Heindl, Paehl, DE;
Gisela Betzl, Andechs, DE;
Rainer Schmuck, Benediktheuern, DE;
Bernd Schneidinger, Wolfratshausen, DE;
Jessica Strey, Penzberg, DE;
Thomas Meier, Munich, DE;
Waltraud Ankenbauer, Penzberg, DE;
Annette Deufel, Munich, DE;
Dieter Heindl, Paehl, DE;
Gisela Betzl, Andechs, DE;
Rainer Schmuck, Benediktheuern, DE;
Bernd Schneidinger, Wolfratshausen, DE;
Jessica Strey, Penzberg, DE;
Roche Diagnostics Operations, Inc., Indianapolis, IN (US);
Abstract
It was found that a fragment of nativeDNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.