The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 07, 2011
Filed:
May. 29, 2009
Werner Hoelke, Penzberg, DE;
Hellmut Eckstein, Weilheim, DE;
Michaela Fischer, Wolfratshausen, DE;
Antje Liehre, Penzberg, DE;
Bernhard Suppmann, Weilheim, DE;
Johann-peter Thalhofer, Weilheim, DE;
Werner Hoelke, Penzberg, DE;
Hellmut Eckstein, Weilheim, DE;
Michaela Fischer, Wolfratshausen, DE;
Antje Liehre, Penzberg, DE;
Bernhard Suppmann, Weilheim, DE;
Johann-Peter Thalhofer, Weilheim, DE;
Roche Diagnostics Operations, Inc., Indianapolis, IN (US);
Abstract
The present invention provides a method for purifyingcollagenase type I and type II proteins from a complex mixture by subsequently performing a precipitation with ammonium sulfate, hydrophobic interaction chromatography, cation exchange chromatography, and anion chromatography. Conditions are provided which lead to a stabilized, partially purified preparation even after the precipitation step. The method of the invention leads to a quick and efficient removal of other proteolytic activities. The preparations according to the invention provide exceptionally pure and intact collagenase type I and type II proteins which are enzymatically active. The invention also provides blends of the two isolated proteins. The invention further provides the use of the purified collagenase proteins or blends thereof for treating a tissue sample in vitro.