The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 24, 2011
Filed:
Nov. 23, 2009
Changshen Liu, Shanghai, CN;
Jun Lin, Shanghai, CN;
Jiangchao Qian, Shanghai, CN;
Yuan Yuan, Shanghai, CN;
Changshen Liu, Shanghai, CN;
Jun Lin, Shanghai, CN;
Jiangchao Qian, Shanghai, CN;
Yuan Yuan, Shanghai, CN;
Shanghai Rebone Biomaterials Co., Ltd., Shanghai, CN;
Abstract
The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on theexpression system and a method for the preparation of the rhBMP-2. Specifically, the invention provides the optimal DNA sequences suitable forexpression system, the methods for efficient preparation of the rhBMP-2, and the related construction of the recombinant bacteria, the expression and purification technologies. Compared with the traditional hBMP-2 gene without optimizing, the rhBMP-2 expression level of the optimized gene inis increased by 50%. Additionally, this invention also provides a method for preparation long chain rhBMP-2 with enhanced renaturation efficiency and yield of purification.