The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 26, 2011
Filed:
May. 04, 2005
Michel Bouvier, Montreal, CA;
Pascale Charest, San Diego, CA (US);
Michel Bouvier, Montreal, CA;
Pascale Charest, San Diego, CA (US);
Valorisation-Recherche, Limited Partnership, Montreal, Quebec, CA;
Abstract
An intramolecular bioluminescence resonance energy transfer (BRET), biosensor for monitoring receptor activity and signalling cascades is disclosed. The 'double-brilliance' biosensor sandwiches β-arrestin (β-arr) betweenluciferase (Luc) and the yellow fluorescent protein (YFP). β-arr associates with G-protein coupled receptors GPCR following receptor activation, bringing Luc and YPF into close proximity that favours energy transfer. In addition to providing new insights into the agonist-induced conformational rearrangements of β-arr in living cells, the double-brilliance β-arr offers a universal biosensor for GPCR activation, allowing the study of native receptors in large-scale screening analysis. The activity of other signalling molecules known to interact with β arrestin could also be monitored by double brilliance β arr.