The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 15, 2011

Filed:

Oct. 26, 1999
Applicants:

David J Squirrell, Salisbury, GB;

Melenie J Murphy, Salisbury, GB;

Rachel L Price, Salisbury, GB;

Christopher R Lowe, Cambridge, GB;

Peter J White, Cambridge, GB;

Laurence C Tisi, Cambridge, GB;

James a H Murray, Cambridge, GB;

Inventors:

David J Squirrell, Salisbury, GB;

Melenie J Murphy, Salisbury, GB;

Rachel L Price, Salisbury, GB;

Christopher R Lowe, Cambridge, GB;

Peter J White, Cambridge, GB;

Laurence C Tisi, Cambridge, GB;

James A H Murray, Cambridge, GB;

Assignee:

Promega Corporation, Madison, WI (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); C12N 15/00 (2006.01); C12N 1/20 (2006.01); C12N 5/04 (2006.01); C07H 21/04 (2006.01); A01H 9/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

A protein having luciferase activity and at least 60% similarity to luciferase fromororwherein in the sequence of the enzyme, at least one of (a) the amino acid residue corresponding to residue 214 inluciferase; (b) the amino acid residue corresponding to residue 232 inluciferase; (c) the amino acid residue corresponding to residue 295 inluciferase; (d) the amino acid residue corresponding to acid 14 ofluciferase; (e) the amino acid residue corresponding to amino acid 35 ofluciferase; (f) the amino acid residue corresponding to amino acid residue 105 ofluciferase; (g) the amino acid residue corresponding to amino acid residue 234 ofluciferase; (h) the amino acid residue corresponding to amino acid residue 420 ofluciferase; (i) the amino acid residue corresponding to amino acid residue 310 ofluciferase; is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.


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