The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2011
Filed:
Aug. 16, 2005
Xiyu Jia, Newport Beach, CA (US);
Jan Kostal, Saint Paul, MN (US);
Jonathan Anthony Claypool, Seattle, WA (US);
Xiyu Jia, Newport Beach, CA (US);
Jan Kostal, Saint Paul, MN (US);
Jonathan Anthony Claypool, Seattle, WA (US);
Zymo Research Corporation, Irvine, CA (US);
Abstract
The present invention relates to the simple, gentle, and efficient extraction of biological material from(). The use ofin research laboratories depends on the ability to prepare lysates to isolate the desired products under investigation. The present invention includes methods and engineeredstrains that are capable of rapid controlled lysis or herein 'autolysis'. The XJa strains were made from JM109 and the XJb strains from BL21 by insertion of the λ R or (λ SR) lytic endolysin gene to replace the tightly regulated araB gene. Thus, arabinose becomes a non-metabolizable inducer and the controlled autolysis phenotype is induced by the Ppromoter by the presence of saturating arabinose. Upon induction of the bacteriophage λR endolysin, theremains intact but is efficiently lysed after one freeze-thaw cycle. The present invention is usable with many different buffer systems and is flexible in this regard. The controlled autolysis phenotype shows increased yields and purity of extracted protein compared to detergent based lysis or traditional sonication lysis methods. The present invention is useful for routine protein expression or nucleic acid extraction and also for high-through-put manipulation involving protein or nucleic acid from