The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2011
Filed:
May. 05, 2008
Applicants:
Paul Mitsis, Trenton, NJ (US);
Anuradha Sekher, Belle Mead, NJ (US);
Gyanendra Kumar, Franklin Park, NJ (US);
Inventors:
Paul Mitsis, Trenton, NJ (US);
Anuradha Sekher, Belle Mead, NJ (US);
Gyanendra Kumar, Franklin Park, NJ (US);
Assignee:
GE Healthcare Bio-Sciences Corp., Piscataway, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
Abstract
The present invention provides methods, formulation and kits for the synthesis of long nucleic acid fragments. An improved PCR method is provided for amplifying long DNA fragments. In particular, a single thermostable DNA polymerase is used for the rapid amplification of over 10 kb long DNA fragments. Also provided is a method for extending long complementary DNA strands using this single enzyme formulation.