The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 18, 2011
Filed:
Mar. 31, 2006
Christine Kurata, LaJolla, CA (US);
Daniel Capaldi, Carlsbad, CA (US);
Zhiwei Wang, Carlsbad, CA (US);
Nhuy Luu, Vista, CA (US);
Hans-joachim Gaus, Carlsbad, CA (US);
Claus André Frank Rentel, San Marcos, CA (US);
Christine Kurata, LaJolla, CA (US);
Daniel Capaldi, Carlsbad, CA (US);
Zhiwei Wang, Carlsbad, CA (US);
Nhuy Luu, Vista, CA (US);
Hans-joachim Gaus, Carlsbad, CA (US);
Claus André Frank Rentel, San Marcos, CA (US);
Isis Pharmaceuticals, Inc., Carlsbad, CA (US);
Abstract
Provided is a method comprising introducing a sample comprising a plurality of oligonucleotides into an ion pair high performance liquid chromatography column having a buffered mobile phase and allowing at least a portion of the oligonucleotides to separate; allowing the oligonucleotides to elute from the column; and introducing the oligonucleotides into a mass spectrometer and quantifying at least a portion of the oligonucleotides by mass spectrometry. In the method, at least a portion of the oligonucleotides are co-eluting oligonucleotides that differ in mass by no more than 20%; and the buffered mobile phase causes at least 50 mole percent of co-eluting oligonucleotides to have the same charge when they enter the mass spectrometer.