The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 28, 2010
Filed:
Dec. 12, 2005
Brian J. Reardon, Seattle, WA (US);
Susan H. Julien, Seattle, WA (US);
Chung-leung Chan, Sammamish, WA (US);
Hong Y. Liu, Seattle, WA (US);
Brian J. Reardon, Seattle, WA (US);
Susan H. Julien, Seattle, WA (US);
Chung-leung Chan, Sammamish, WA (US);
Hong Y. Liu, Seattle, WA (US);
ZymoGenetics, Inc., Seattle, WA (US);
Abstract
The expression vectors and methods using anexpression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in. Using the expression vectors, the FGF18 gene was produced into a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientstrains, as well as OmpT and fhuA negative strains transformed with an FGF18 expression vector.