The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 14, 2010
Filed:
Dec. 12, 2005
John A. Howe, Greenbrook, NJ (US);
Ken N. Wills, Carlsbad, CA (US);
Robert Orville Ralston, Ii, Union, NJ (US);
Scott Joseph Sherrill, Chesterfield, MO (US);
John A. Howe, Greenbrook, NJ (US);
Ken N. Wills, Carlsbad, CA (US);
Robert Orville Ralston, II, Union, NJ (US);
Scott Joseph Sherrill, Chesterfield, MO (US);
Schering Corporation, Kenilworth, NJ (US);
Abstract
The present invention provides cell lines for the production of E1-deleted adenovirus (rAd) vectors that complement E1A and E1B functions. The present invention also provides cell lines for the production of E1- and E2-deleted adenovirus vectors that complement E1A, E1B and E2B polymerase functions. The invention provides particular cell lines that complement E1A function by insertion of an E1A sequence containing mutations in the 243R and 289R proteins and an E1B sequence comprising the E1B-55K gene. Production yields in the resulting producer cell lines, designated SL0003 and SL0006, were similar to those obtained from 293 cells without generation of detectable recombinant replication competent adenovirus ('RCA').