The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 7846658 B1

PDF
Full Text
Expired
Date of Patent:
Dec. 07, 2010

Filed:

Sep. 29, 2004
Applicant:

Ville Laitala, Turku, FI;

Inventor:

Ville Laitala, Turku, FI;

Assignee:

Wallac Oy, Turku, FI;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 33/53 (2006.01); C12M 1/38 (2006.01);
U.S. Cl.
CPC ...
Abstract

The invention relates to a method for improving the detection sensitivity in homogenous TR-FRET based bioaffinity assays. The sensitivity is improved by the use of long lifetime donors together with a high energy transfer efficiency and by carrying out the detection of the energy transfer based emission of the acceptor in a time window which is opened after a delay of 1 microsecond or more, but less than 50 microseconds, calculated from the donor excitation, and which time window has a width of 1 microsecond or more, but less than 100 microseconds. The invention concerns also the use of the improved method in multianalyte assays. Furthermore, the invention concerns a device suitable for carrying out the improved method.


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