The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 19, 2010

Filed:

Apr. 20, 2007
Applicants:

Rie Nagai, Tsuruga, JP;

Masao Kitabayashi, Tsuruga, JP;

Yoshiaki Nishiya, Tsuruga, JP;

Inventors:

Rie Nagai, Tsuruga, JP;

Masao Kitabayashi, Tsuruga, JP;

Yoshiaki Nishiya, Tsuruga, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/00 (2006.01); C12N 9/78 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

To overcome disadvantages of a known creatinine amide hydrolase, and provide a creatinine amide hydrolase having improved affinity for creatinine or having a decreased Km value for creatinine, and also provide a reagent composition for use in the determination of creatinine, which is adapted to an automated analysis apparatus and is excellent in accuracy, preciseness and economic efficiency. Disclosed is a modified creatinine amide hydrolase having improved affinity for a substrate compared to an unmodified one. Also disclosed is a creatinine determination reagent comprising the modified creatinine amide hydrolase, a creatinine amidino hydrolase, sarcosine oxidase and a reagent for detection of hydrogen peroxide.


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