The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 31, 2010
Filed:
Jan. 11, 2007
Peter C. Simons, Albuquerque, NM (US);
Larry A. Sklar, Albuquerque, NM (US);
Eric R. Prossnitz, Albuquerque, NM (US);
Angela Wandinger-ness, Albuquerque, NM (US);
Mathewos Z. Tessema, Albuquerque, NM (US);
John C. Reed, Ranch Santa Fe, CA (US);
Dayong Zhai, San Diego, CA (US);
Peter C. Simons, Albuquerque, NM (US);
Larry A. Sklar, Albuquerque, NM (US);
Eric R. Prossnitz, Albuquerque, NM (US);
Angela Wandinger-Ness, Albuquerque, NM (US);
Mathewos Z. Tessema, Albuquerque, NM (US);
John C. Reed, Ranch Santa Fe, CA (US);
Dayong Zhai, San Diego, CA (US);
STC.UNM, Albuquerque, NM (US);
Sanford-Burnham Medical Research Institute, La Jolla, CA (US);
Abstract
The present invention relates generally to glutathione derivatized beads which are adapted for use in conjunction with glutathione-S-transferase fusion proteins (generally, GST fusion proteins, which contain a fluorescent label such as fluorescent green protein) for use in flow cytometry. The present invention also relates to methods for detecting and/or quantifying interactions between a GST fusion protein and their binding partners, in particular, labeled binding partners such as fluorescently labeled binding partners. By creating glutathione beads with an appropriate high or increased site density, disadvantages often associated with low affinity systems and quick off-rates in solution may be resolved to provide a workable system and method. Methods of identifying potential agonists, antagonists and regulator compounds of proteins fused to GST from libraries of compounds represents another aspect of the present invention.