The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 17, 2010
Filed:
Feb. 27, 2009
Michael J. Betenbaugh, Baltimore, MD (US);
Shawn Lawrence, Dobbs Ferry, NY (US);
Yuan C. Lee, Timonium, MD (US);
Timothy A. Coleman, Gaithersburg, MD (US);
Michael J. Betenbaugh, Baltimore, MD (US);
Shawn Lawrence, Dobbs Ferry, NY (US);
Yuan C. Lee, Timonium, MD (US);
Timothy A. Coleman, Gaithersburg, MD (US);
The John Hopkins University, Baltimore, MD (US);
Human Genome Sciences, Inc., Rockville, MD (US);
Abstract
Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.