The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 04, 2010
Filed:
Apr. 25, 2003
Akihiro Hino, Tsukuba, JP;
Takeshi Matsuoka, Tsukuba, JP;
Hideo Kuribara, Tsukuba, JP;
Tomoaki Yoshimura, Moriya, JP;
Yoichiro Shindo, Moriya, JP;
Satoshi Futo, Zama, JP;
Machiko Ogawa, Sagamihara, JP;
Akihiro Hino, Tsukuba, JP;
Takeshi Matsuoka, Tsukuba, JP;
Hideo Kuribara, Tsukuba, JP;
Tomoaki Yoshimura, Moriya, JP;
Yoichiro Shindo, Moriya, JP;
Satoshi Futo, Zama, JP;
Machiko Ogawa, Sagamihara, JP;
National Food Research Institute, Ibaraki, JP;
Asahi Breweries, Ltd., Tokyo, JP;
Abstract
The present invention relate to a method of detecting a genetic recombinant by using the PCR method. A method of quantitatively detecting method is provided whereby the total content ratio of the genetic recombinants and the individual content ratio of the genetic recombinant in a population containing plural genetic recombinant lines can be quantified. The method of the present invention comprises performing PCR for the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species corresponding to the recombinant using, as a standard molecule, a molecule containing the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species on the single molecule, and determining the content ratio of the number of molecules thereof.