The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 20, 2010
Filed:
Jul. 18, 2003
George Tzertzinis, Cambridge, MA (US);
George Feehery, West Newbury, MA (US);
Christopher Noren, Boxford, MA (US);
Corinna Tuckey, San Francisco, CA (US);
Larry Mcreynolds, Beverly, MA (US);
Yinhua Zhang, North Reading, MA (US);
George Tzertzinis, Cambridge, MA (US);
George Feehery, West Newbury, MA (US);
Christopher Noren, Boxford, MA (US);
Corinna Tuckey, San Francisco, CA (US);
Larry McReynolds, Beverly, MA (US);
Yinhua Zhang, North Reading, MA (US);
New England Biolabs, Inc., Ipswich, MA (US);
Abstract
A method for obtaining a mixture of heterogenous short double-stranded RNA molecules suitable for use in gene silencing (hsiRNA) by subjecting large double-stranded RNA to enzymatic cleavage under specified conditions. The resulting mixture consistently includes enhanced representation of fragments having a size of 21-22 nucleotides absent any fractionation step. The fragments contain sequences that collectively span the entire length of the large double-stranded RNA from which they are derived. Double-stranded RNA with sequences that individually represent segments of a target mRNA may be analyzed using the methods described herein to identify the most active subset of hsiRNA fragments or individual siRNA fragments for achieving gene silencing for any gene or transcribed sequences. A method is additionally provided for preparing and cloning DNA encoding selected siRNA, hsiRNA mixtures or hairpin sequences to provide a continuous supply of a gene silencing reagent derived from any long double-stranded RNA.