The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2009
Filed:
Mar. 31, 2005
Randall L. Kincaid, Rockville, MD (US);
George Oyler, Baltimore, MD (US);
Yen Che Tsai, Baltimore, MD (US);
Paul S. Fishman, Baltimore, MD (US);
Randall L. Kincaid, Rockville, MD (US);
George Oyler, Baltimore, MD (US);
Yen Che Tsai, Baltimore, MD (US);
Paul S. Fishman, Baltimore, MD (US);
Other;
Abstract
A system and method for identifying aneurotoxin inhibitor employing aneurotoxin substrate complex having a peptide substrate, preferably SNAP-25, a reporter domain on one side of said peptide substrate and an immobilization domain on the opposite side of said peptide substrate. Theneurotoxin inhibitor is identified by its ability to decrease the relative amount of cleaved complex, detected through measuring a decrease in complex bound to a solid support. The method of the present invention also utilizes novel cells that express aneurotoxin substrate complex. The methods of the present invention are adapted for cell based screening to monitor the catalytic activity of a BoNT in living cells and to identify molecules that inhibit the catalytic activity of a BoNT in living cells. Also provided are novel stable cell lines that express thetoxin substrate complex and viral vectors capable of efficiently expressing an active light chain of the BoNT within mammalian cells.