The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 20, 2009
Filed:
Feb. 25, 2003
Masayori Inouye, New Brunswick, NJ (US);
Sangita Phadtare, Highland Park, NJ (US);
Bing Xia, Newton, MA (US);
Guoliang Qing, Piscataway, NJ (US);
Haiping KE, Highland Park, NJ (US);
Masayori Inouye, New Brunswick, NJ (US);
Sangita Phadtare, Highland Park, NJ (US);
Bing Xia, Newton, MA (US);
Guoliang Qing, Piscataway, NJ (US);
Haiping Ke, Highland Park, NJ (US);
Takara Bio, Inc., Shiga, JP;
Abstract
The present invention relates to a DNA molecule or vector and a host cell containing this DNA molecule or vector which can be used to produce a heterologous polypeptide under conditions that elicit a cold shock response in the host cell. The DNA molecule and vector include a nucleotide sequence encoding a heterologous polypeptide and a promoter and 5'-UTR from a cold shock inducible gene which directs its expression. In addition, an AT-rich sequence that enhances translation under cold shock inducible conditions is either present in the coding sequence of the heterologous polypeptide or in an additional element inserted between the coding sequence and the cold shock inducible promoter and 5′-UTR.