The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 18, 2009
Filed:
Sep. 21, 2004
Caifu Chen, Palo Alto, CA (US);
Dana Ridzon, San Ramon, CA (US);
Zhaohui Zhou, Fremont, CA (US);
Kai Qin Lao, Pleasanton, CA (US);
Neil A. Straus, Emeryville, CA (US);
Caifu Chen, Palo Alto, CA (US);
Dana Ridzon, San Ramon, CA (US);
Zhaohui Zhou, Fremont, CA (US);
Kai Qin Lao, Pleasanton, CA (US);
Neil A. Straus, Emeryville, CA (US);
Applied Biosystems, LLC, Carlsbad, CA (US);
Abstract
The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3' target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.