The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 04, 2009
Filed:
Jun. 15, 2004
Michiyo Yanase, Kobe, JP;
Hiroki Takata, Kobe, JP;
Kazutoshi Fujii, Suita, JP;
Takeshi Takaha, Kobe, JP;
Takashi Kuriki, Suita, JP;
Michiyo Yanase, Kobe, JP;
Hiroki Takata, Kobe, JP;
Kazutoshi Fujii, Suita, JP;
Takeshi Takaha, Kobe, JP;
Takashi Kuriki, Suita, JP;
Ezaki Glico Co., Ltd., Osaka, JP;
Abstract
An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to positionin a motif sequenceL orH, a position corresponding to positionin a motif sequence, and a position corresponding to positionin a motif sequenceL orH, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.