The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 30, 2009
Filed:
Aug. 17, 2006
Tatsuki Kashiwagi, Kawasaki, JP;
Nobuhisa Shimba, Kawasaki, JP;
Kohki Ishikawa, Kawasaki, JP;
Ei-ichiro Suzuki, Kawasaki, JP;
Keiichi Yokoyama, Kawasaki, JP;
Kazuo Hirayama, Kawasaki, JP;
Tatsuki Kashiwagi, Kawasaki, JP;
Nobuhisa Shimba, Kawasaki, JP;
Kohki Ishikawa, Kawasaki, JP;
Ei-ichiro Suzuki, Kawasaki, JP;
Keiichi Yokoyama, Kawasaki, JP;
Kazuo Hirayama, Kawasaki, JP;
Ajinomoto Co., Inc., Tokyo, JP;
Abstract
The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from(MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.