The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 17, 2009
Filed:
Sep. 30, 2003
Richard Thompson, Baltimore, MD (US);
Michele Cramer, Baltimore, MD (US);
Carol Ann Fierke, Ann Harbor, MI (US);
Hui Hui Zeng, Ellicott City, MD (US);
Rebecca Bozym, Moon Township, PA (US);
Richard Thompson, Baltimore, MD (US);
Michele Cramer, Baltimore, MD (US);
Carol Ann Fierke, Ann Harbor, MI (US);
Hui Hui Zeng, Ellicott City, MD (US);
Rebecca Bozym, Moon Township, PA (US);
Other;
Abstract
A highly selective and sensitive of a carbonic anhydrase-based method for measurement of zinc ion by an excitation ratiometric format based on resonance energy transfer: i.e., where the zinc ion level is transduced as the ratio of fluorescence intensities excited at two different excitation wavelengths, is provided. The method can be used very well in a fluorescence microscopy format. A detection limit of about 10 pM in zinc buffered systems, a ten to one thousand-fold improvement on the Fura indicators (which respond to Ca and Mg as well), and a one hundred thousand-fold improvement on the recently described FuraZin-1 is achieved.