The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 06, 2009
Filed:
Sep. 08, 2005
Takahiro Kikawada, Tsukuba, JP;
Takashi Okuda, Tsukuba, JP;
Masahiko Watanabe, Tsukuba, JP;
Kazuei Mita, Tsukuba, JP;
Keiko Kadono, Tsukuba, JP;
Takahiro Kikawada, Tsukuba, JP;
Takashi Okuda, Tsukuba, JP;
Masahiko Watanabe, Tsukuba, JP;
Kazuei Mita, Tsukuba, JP;
Keiko Kadono, Tsukuba, JP;
National Institute of Agrobiological Sciences, Ibaraki, JP;
Abstract
An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced fromlarvae in a desiccated state, aEST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins. When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from these genes were heat soluble even when boiling, so that these proteins have hydrophilic property as high as plant LEA proteins. Therefore, the three isolated genes were found to be novel-derived LEA genes. Furthermore, introduction of these genes into animal cells successfully conferred desiccation resistance to the cells.