The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 16, 2008
Filed:
Jul. 07, 2004
Andrew Berlin, San Jose, CA (US);
Steven J. Kirch, Pleasanton, CA (US);
Gabi Neubauer, Los Gatos, CA (US);
Valluri Rao, Saratoga, CA (US);
Mineo Yamakawa, Campbell, CA (US);
Andrew Berlin, San Jose, CA (US);
Steven J. Kirch, Pleasanton, CA (US);
Gabi Neubauer, Los Gatos, CA (US);
Valluri Rao, Saratoga, CA (US);
Mineo Yamakawa, Campbell, CA (US);
Intel Corporation, Santa Clara, CA (US);
Abstract
The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.