The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 02, 2008
Filed:
Mar. 21, 2005
Toshiki Tamura, Tsukuba, JP;
Hideki Sezutsu, Tsukuba, JP;
Isao Kobayashi, Tsukuba, JP;
Katsura Kojima, Tsukuba, JP;
Toshio Kanda, Tsukuba, JP;
Keiro Uchino, Tsukuba, JP;
Toshiki Tamura, Tsukuba, JP;
Hideki Sezutsu, Tsukuba, JP;
Isao Kobayashi, Tsukuba, JP;
Katsura Kojima, Tsukuba, JP;
Toshio Kanda, Tsukuba, JP;
Keiro Uchino, Tsukuba, JP;
National Institute of Agrobiological Sciences, Ibaraki, JP;
Abstract
Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of the silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silkgland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amounts of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk glands. Furthermore, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.