The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 08, 2008
Filed:
Oct. 13, 2004
Hans Berg, Uppsala, SE;
David Buckley, Uppsala, SE;
Maria Brännholm, Uppsala, SE;
Anders Hagvall, Uppsala, SE;
Henrik Ihre, Sundbyberg, SE;
Eva Holmgren, Uppsala, SE;
Anders Larsson, Bromma, SE;
Dag Lindstrom, Vattholma, SE;
Hans Berg, Uppsala, SE;
David Buckley, Uppsala, SE;
Maria Brännholm, Uppsala, SE;
Anders Hagvall, Uppsala, SE;
Henrik Ihre, Sundbyberg, SE;
Eva Holmgren, Uppsala, SE;
Anders Larsson, Bromma, SE;
Dag Lindstrom, Vattholma, SE;
GE Healthcare Bio-Sciences AB, Uppsala, SE;
Abstract
The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.