The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 13, 2008

Filed:

Apr. 30, 2002
Applicants:

Hans-ulrich Koop, München, DE;

Stefan Mühlbauer, Freising, DE;

Sebastian Klaus, Gräfelfing, DE;

Christian Eibl, Ismaning, DE;

Fong-chin Huang, Taipei, TW;

Timothy J. Golds, Freising, DE;

Inventors:

Hans-Ulrich Koop, München, DE;

Stefan Mühlbauer, Freising, DE;

Sebastian Klaus, Gräfelfing, DE;

Christian Eibl, Ismaning, DE;

Fong-Chin Huang, Taipei, TW;

Timothy J. Golds, Freising, DE;

Assignee:

Icon Genetics AG, Munich, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
Abstract

A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.


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