The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 11, 2008
Filed:
Mar. 01, 2006
Prabha Devi, Goa, IN;
Chandrakant Govind Naik, Goa, IN;
Solimabi Wahidulla, Goa, IN;
Lisette D'souza, Goa, IN;
Ely Rodrigues, Goa, IN;
Asha Peketi, Goa, IN;
Prabha Devi, Goa, IN;
Chandrakant Govind Naik, Goa, IN;
Solimabi Wahidulla, Goa, IN;
Lisette D'Souza, Goa, IN;
Ely Rodrigues, Goa, IN;
Asha Peketi, Goa, IN;
Council of Scientific and Industrial Research, New Delhi, IN;
Abstract
The present invention relates to a plant mangrove-associated fungus, and a simple and efficient method of obtaining high yield of pure mannitol from a plant mangrove-associated fungus, said method comprising steps of cutting the leaves into small pieces and placing it on potato dextrose agar (PDA) plates for 48 hours, maintaining the culture at temperature ranging between 26-47° C. and salinity ranging between 4-32 ppt with occasional stress conditions for about 17-19 days to obtain mycelial mat, sonicating the mat to lyse the cells, extracting crude from the sonicated mat using methanol repeatedly, concentrating the crude, treating the concentrated crude extract with solvents of increasing polarity, obtaining aqueous fraction as white powder residue after the treatment containing crude mannitol, purifying the crude mannitol by chromatography to obtain pure mannitol with about 75% of the total crude extract.