The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 11, 2008
Filed:
Sep. 09, 2003
Sean C. Semple, Vancouver, CA;
Sandra K. Klimuk, N. Vancouver, CA;
Troy Harasym, Vancouver, CA;
Michael J. Hope, Vancouver, CA;
Steven M. Ansell, Vancouver, CA;
Pieter Cullis, Vancouver, CA;
Peter Scherrer, Vancouver, CA;
Dan Debeyer, Vancouver, CA;
Sean C. Semple, Vancouver, CA;
Sandra K. Klimuk, N. Vancouver, CA;
Troy Harasym, Vancouver, CA;
Michael J. Hope, Vancouver, CA;
Steven M. Ansell, Vancouver, CA;
Pieter Cullis, Vancouver, CA;
Peter Scherrer, Vancouver, CA;
Dan Debeyer, Vancouver, CA;
The University of British Columbia, Vancouver, CA;
Abstract
Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50%.