The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 26, 2008
Filed:
Aug. 25, 2005
Denis Drapeau, Salem, NH (US);
Yen-tung Luan, Chelmsford, MA (US);
James R. Mercer, Derry, NH (US);
Wenge Wang, North Chelmsford, MA (US);
Daniel R. Lasko, Medford, MA (US);
Denis Drapeau, Salem, NH (US);
Yen-Tung Luan, Chelmsford, MA (US);
James R. Mercer, Derry, NH (US);
Wenge Wang, North Chelmsford, MA (US);
Daniel R. Lasko, Medford, MA (US);
Wyeth Research Ireland Limited, Little Connell, Newbridge, County Kildere, IE;
Abstract
An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.