The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 15, 2008

Filed:

Sep. 26, 2003
Applicants:

Laurie Engel, DeForest, WI (US);

John W. Schultz, Verona, WI (US);

Tonny M. Johnson, Madison, WI (US);

Kristopher Zimmerman, Madison, WI (US);

Laura L. Bozek, Madison, WI (US);

Judith N. Stevens, Oregon, WI (US);

Inventors:

Laurie Engel, DeForest, WI (US);

John W. Schultz, Verona, WI (US);

Tonny M. Johnson, Madison, WI (US);

Kristopher Zimmerman, Madison, WI (US);

Laura L. Bozek, Madison, WI (US);

Judith N. Stevens, Oregon, WI (US);

Assignee:

Promega Corporation, Madison, WI (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12P 21/04 (2006.01); A23J 1/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.


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