The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 02, 2007

Filed:

Nov. 28, 2000
Applicants:

Peter Palese, Leonia, NJ (US);

Mark Krystal, Westport, CT (US);

Jeffrey Parvin, Lincoln, MA (US);

Inventors:

Peter Palese, Leonia, NJ (US);

Mark Krystal, Westport, CT (US);

Jeffrey Parvin, Lincoln, MA (US);

Assignee:

MedImmune Vaccines, Inc., Mountain View, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 15/63 (2006.01); C12N 15/11 (2006.01); C12N 15/85 (2006.01); C12N 15/86 (2006.01); C07H 15/11 (2006.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Recombinant negative-strand viral RNA templates are described which may be used with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA polymerase. The resulting RNA templates are of the negative-polarity and contain appropriate terminal sequences which enable the viral RNA-synthesizing apparatus to recognize the template. Bicistronic mRNAs can be constructed to permit internal initiation of translation of viral sequences and allow for the expression of foreign protein coding sequences from the regular terminal initiation site, or vice versa.


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