The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 03, 2007

Filed:

Mar. 10, 2005
Applicants:

Seiichi Toki, Ibaraki, JP;

Hiroaki Ichikawa, Ibaraki, JP;

Hidemitsu Nakamura, Ibaraki, JP;

Kiyoshi Kawai, Tokyo, JP;

Koichiro Kaku, Tokyo, JP;

Tsutomu Shimizu, Tokyo, JP;

Inventors:

Seiichi Toki, Ibaraki, JP;

Hiroaki Ichikawa, Ibaraki, JP;

Hidemitsu Nakamura, Ibaraki, JP;

Kiyoshi Kawai, Tokyo, JP;

Koichiro Kaku, Tokyo, JP;

Tsutomu Shimizu, Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C12N 5/05 (2006.01); C12N 15/09 (2006.01); A01H 1/00 (2006.01); A01H 5/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5×SSC at 42° C.; The expression vector, also, includes a coding sequence located downstream of the promoter. Additionally, the method includes introducing the expression vector into a plant cell to obtain a transgenic plant; wherein the promoter activity is lower in mature seeds or root bases than in plant tissues other than mature seeds or root bases. The present invention also provides transformants containing a promoter; the promoter includes any of the DNAs (a) to (c) described above.


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