The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 17, 2007
Filed:
Oct. 09, 2003
Daniel Buttry, Laramie, WY (US);
Angie M. Oppedahl, Boone, IA (US);
Steven J. Lasky, Ankeny, IA (US);
Edward A. Orr, The Woodlands, TX (US);
Daniel Buttry, Laramie, WY (US);
Angie M. Oppedahl, Boone, IA (US);
Steven J. Lasky, Ankeny, IA (US);
Edward A. Orr, The Woodlands, TX (US);
Advanced Analytical Technologies, Inc., Ames, IA (US);
Abstract
This invention relates to methods for reducing background fluorescence in cell detection and measurement systems. In accordance with one aspect of the invention, cell-impermeant chemical agents are used to modify the fluorescent probes that reside outside the cells to be detected. The chemical agents can chemically modify one or more functional groups in the probes, thereby reducing or eliminating the fluorescence of the probes. In accordance with another aspect of the invention, Acid Black 48 is used to reduce background fluorescence produced by fluorescent probes such as SYTO 62.