The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 27, 2007
Filed:
May. 30, 2003
Akiko Nishi, Akashi, JP;
Takumi Mano, Tatsuno, JP;
Shinichi Yokota, Kakogawa, JP;
Masayuki Takano, Kakogawa, JP;
Kazuyoshi Yajima, Akashi, JP;
Akiko Nishi, Akashi, JP;
Takumi Mano, Tatsuno, JP;
Shinichi Yokota, Kakogawa, JP;
Masayuki Takano, Kakogawa, JP;
Kazuyoshi Yajima, Akashi, JP;
Kaneka Corporation, Osaka, JP;
Abstract
The subject of the present invention is to provide a β-lactam acylase protein having high activity, a gene coding for said β-lactam acylase protein, a recombinant vector having said gene, a transformant containing said recombinant vector, and a method of producing a β-lactam antibiotic such as amoxycillin using said β-lactam acylase. A β-lactam acylase gene ofwas cloned, the DNA base sequence and the amino acid sequence expected therefrom was determined, and aβ-lactam acylase gene was obtained. This gene was found to code for a protein with a molecular weight of about 70 kDa and having β-lactam acylase activity, and could efficiently produce amoxycillin without being inhibited by phenylacetic acid, etc. Furthermore, by modification of the amino acid sequence, a protein which can more efficiently produce amoxycillin could be obtained.