The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 23, 2007
Filed:
May. 22, 2003
Brian M. Beyer, Lincroft, NJ (US);
Bruce A. Malcolm, Westfield, NJ (US);
Corey O. Strickland, Martinsville, NJ (US);
Wenyan Wang, Edison, NJ (US);
Eileen Wilson, Raritan, NJ (US);
Brian M. Beyer, Lincroft, NJ (US);
Bruce A. Malcolm, Westfield, NJ (US);
Corey O. Strickland, Martinsville, NJ (US);
Wenyan Wang, Edison, NJ (US);
Eileen Wilson, Raritan, NJ (US);
Schering Corporation, Kenilworth, NJ (US);
Abstract
The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of β-secretase (BACE) polypeptide. Polypeptides used herein are derived from human BACE which is also known by the synonyms 'mamapsin 2', 'human β-site APP-cleaving enzyme, and Asp2'. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.